ABSTRACT
Objective:To analyze the inhibiting mechanism of microRNA-199a/b-3p ( miR-199a/b-3p) on cell motility of breast cancer cells.Methods:The expression of PAK4 in MDA-MB-231 cells transfected with miR-199a/b-3p was detected by Western blot.The biology behaviors of MDA-MB-231 cells transfected with miR-199a/b-3p or PAK4 SiRNA were analysed by cell migration assay,invasion assay and protrusion dynamics.Results: MiR-199a/b-3p could suppress the expression of PAK 4 in MDA-MB-231 cells.Comparing with normal control ,miR-199a/b-3p or PAK4 SiRNA could suppress the migration ,invasion and membrane protrusion of MDA-MB-231 cells.Conclusion:miR-199a/b-3p could suppress the expression of PAK4,which are considered key breast cancer suppressors and inhibit the cell motility of breast cancer cells.
ABSTRACT
Objective:To analyze the effect of PAK4 interruption by microRNA-199a/b-3p (miR-199a/b-3p) on migration and invasion of hepatocellular carcinoma (HCC).Methods: To test targeting of PAK4 by miR-199a/b-3p,we used luciferase assay in HEK293T cells cotransfected miR-199a/b-3p mimcs and pmirGLO-PAK4 3′UTR.The expression of PAK4 in SMMC-7721 transfected with miR-199a/b-3p was detected by Western blot.The biology behaviors of SMMC-7721 cells transfected with miR-199a/b-3p or PAK4 Si were analysed by cell migration assay and invasion assay.Results:MiR-199a/b-3p could suppress the mRNA and protein ex-pression of PAK4 by targeting PAK4 3′UTR,and the downregulating PAK 4 expression suppress the migration and invasion of SMMC-7721 cells.Conclusion: MiR-199a/b-3p could suppress the expression of PAK 4, which are considered key HCC suppressors and inhibit the migration and invasion of HCC cells.
ABSTRACT
Objective To establish the quality standard for Fushenning Tablets. Methods Radix et Rhizoma Rhei,Fructus Gardeniae,Rhizoma Anemarrhenae,Radix Stephaniae Tetrandrae were identified by TLC,and the content of berberine hydrochloride of Cortex Phellodendri Chinensis was determined by HPLC. Results TLC could identify Radix et Rhizoma Rhei,Fructus Gardeniae,Rhizoma Anemarrhenae,Radix Stephaniae Tetrandrae effectively. Berberine Hydrochloride showed a good linear relationship in the range of 0.021 42～ 0.110 20 ? g. The average recovery was 100.6 %(n=6) and RSD was1.4 % . Conclusion This method is simple,accurate,specific and reproducible,and can be used for quality control of Fushenning Tablets.
ABSTRACT
Objective: To establish the determination method of acetamidopyrrolidone in Compound Huonaosu Capsules with RP HPLC. Methods: Kromasil C 18 column was used. The mobile phase was methanol water(10∶90) with 0.6ml?min -1 of flow rate, and the detection wavelength was at 230nm. Results: The linear range of acetamidopyrrolidone concentration was from 0.17 to 0.78mg?ml -1 and correlation coefficient was 0.9995. The average recovery of sample was 99.9% and RSD and 0.62%(n=5). Conclusion: The method is simple, accurate and reproducible. It can be used for determination of acetamidopyrrolidone Compound in Huonaosu Capsules.
ABSTRACT
AIM: To establish the quality standard for Yushangling Capsule(Angelica Sinensis,Borneolum Syntheticum,Radix et Rhizoma Notoginseng,etc.). METHODS: Angelica Sinensis and Borneolum Syntheticum were identified by TLC,and the contents of effective components were determined by HPLC. RESULTS: Angelica sinensis,and Borneolum Syntheticum could be identified by TLC.The linear ranges of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 were 0.104 6-1.987 4 ?g,0.407 6-7.744 4 ?g,0.414 8-7.881 2 ?g,respectively,the average recoveries were 99.7%(RSD=0.6%),99.5%(RSD=0.5%),100.1%(RSD=0.7%),respectively CONCLUSION: The method is simple,accurate and high specificity with good repeatability and can be used for quality control of Yushangling Capsule.